Analytical method development in HPLC
Written by
Srinivas Birudukota
This post is a guide and strategy on how to develop an analytical method in HPLC. It will instruct you on the design of the method, including developing a protocol for sample preparation and analysis. Finally, it will discuss the limitations of HPLC that must be taken into account when developing an analytical method. How does HPLC work? The principle of chromatography uses a moving phase (molecule) to separate two or more stationary phases (molecules). In this case, the stationary phase is a column filled with material that consists of positively charged particles that bind to and immobilize the analyte. The analyte is carried by a mobile phase which makes contact with both stationary phases. The mobile phase can flow back and forth between the two phases based on a specific gradient.
