How to Convert specific optical rotation value to Enantiomer purity Value
In Pharmaceutical compound Enantiomer purity is so much important which can be only identified by Chiral HPLC chromatography (normal phase H!PLC)
the enantiomer or isomers (R-Isomer or S-Isomer) out of two isomers depends on the API only one should be desired and other is undesired.
If we have SOR value of the compound then we can easily calculate the isomer content
Below is the calculation
step-1
SOR/Purity of standard x 100
Step-2
100- step-1 result
Step-3
Step-2 result /2
step-4
S-isomer =step 1 + step 3
R isomer = 100- S isomer
In Pharmaceutical compound Enantiomer purity is so much important which can be only identified by Chiral HPLC chromatography (normal phase H!PLC)
the enantiomer or isomers (R-Isomer or S-Isomer) out of two isomers depends on the API only one should be desired and other is undesired.
If we have SOR value of the compound then we can easily calculate the isomer content
Below is the calculation
step-1
SOR/Purity of standard x 100
Step-2
100- step-1 result
Step-3
Step-2 result /2
step-4
S-isomer =step 1 + step 3
R isomer = 100- S isomer
